中文名称 HAT 检测试剂盒
英文名称 HAT Assay Kit,fluorescent detection
货号 17-289
包装 1kit
价格 7578元
产地 Millipore
说明
Description:              HAT Assay Kit
Key Applications:     ELISA
Application Notes:    Nuclear Extract (source of HAT enzyme activity): Prepare nuclear extracts using a modified protocol of Dignam, et al., Nucleic Acids Res. 22: 1475, 1983 (recommended, but other methods may be used). See citations for reference link. Use 1- 20μl per assay point in the HAT Reaction Cocktail. Optimal nuclear extract concentration will need to be determined empirically.
Species Reactivity:     All
Gene Symbol:           HAT1
Quality Assurance:    Routinely evaluated in a HAT Assay. Biotinylated Histone H4 or Histone H3 peptides were acetylated with recombinant PCAF (14-309) in the presence of Acetyl-CoA and HAT Assay buffer.
Components:
  • Streptavidin Coated Microwell Plate (Cat.# 20-183)
  • Histone H3 Peptide, biotin conjugate (Cat.# 12-403)
  • Histone H4 Peptide, biotin conjugate (Cat.# 12-405)
  • HAT Assay Buffer, 5X (Cat.# 20-148)
  • 07-129
  • TBS, 20X (Cat.# 20-190)
  • 10% BSA in TBS (Cat.# 20-191)
  • Acetyl-Histone H3 (K9, K14) Peptide biotinylated; Histone H4 (Tetra Ac) Peptide biotinylated; Acetyl-CoA; Goat Anti-Rabbit IgG, HRP-conjugate; TMB (tetramethylbenzidine) Substrate Reagent A and TMB (tetramethylbenzidine) Substrate Reagent B
Antibody Category:      Epigenetics & Nuclear Function
Antibody Sub-Category:  Histones
Usage Statement:     Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:     Upstate (Millipore)
Kit or Assay Type:
  • Nuclear Function Kits
  • Enzyme Activity Assays
Host:    Rabbit
Detection Methods:    Fluorescent
Packaging:    Kit capacity: 96 assays