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Product Overview:   CpGenome™ Universal Methylated DNA is enzymatically methylated human male genomic DNA. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.

Materials Provided:    Each vial contains 10 micrograms (100 μl) of human genomic DNA at a concentration of 0.1 μg/μl. Note: this DNA must first be bisulfite modified (S7820) to use a primer that is specific for the methylated form of the gene of interest.

Validation:     Methylation-specific PCR (MSP) was performed on the DNA prior to and post-enzymatic methylation. Sets of primers from the CpG WIZ™ p15 and E-cadherin Amplification Kits were used in the assay. A set of primers designed to anneal to unmethylated DNA and a second set designed to anneal to methylated sequences were used from each kit. The methylated primer sets generated products only after the DNA was methylated.

CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ™ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.

Key Applications:   Software Needed

Application Notes:   For MSP primer design, please use the MethPrime software package. Click here

Species Reactivity:   Human

Usage Statement:    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Presentation:    Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.

Storage Conditions:   Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.

Modifications:   Methylation